Abstract
AIM: To evaluate and compare the gingival and periodontal health status in patients after using Matcha mouthrinse and 2% chlorhexidine (CHX) gluconate mouthwash and also evaluate the efficacy of Matcha extract on Porphyromonas gingivalis (P. gingivalis) and Streptococcus mutans (S. mutans).
MATERIAL AND METHODOLOGY: It is a randomized clinical trial study with 32 adult patients having mild-to-moderate gingivitis and periodontitis. They were divided into two groups, each with 16 patients. Group A was given Matcha extract, and group B was given 2% CHX gluconate. All participants were brought to baseline by oral prophylaxis. They were asked to use their particular group mouth rinse for rinsing twice a day for 1 minute. Indices were taken at baseline, 7, 14, and 28 days. Minimal inhibitory concentration (MIC) was checked for Matcha extract on both P. gingivalis and S. mutans bacteria, and gingival crevicular fluid (GCF) was taken at baseline and after 28 days to check colony-forming units (CFUs) of the samples.
RESULTS: Matcha mouthrinse significantly reduced the inflammatory condition of gingivitis and periodontitis (p < 0.05) and is comparable with 2% CHX gluconate. In vitro evaluation of Matcha extract at various concentrations of MIC shows its effectiveness on P. gingivalis and S. mutans.
CONCLUSION: To conclude, Matcha mouthrinse may be recommended as an alternative mouthrinse for improving oral health status.
CLINICAL SIGNIFICANCE: The findings of this study suggest that Matcha mouthrinse, a natural and antioxidant-rich alternative, is effective in reducing gingival and periodontal inflammation, with outcomes comparable to the gold standard 2% CHX gluconate. Additionally, Matcha extract demonstrated antibacterial efficacy against key periodontal pathogens P. gingivalis and S. mutans. This highlights its potential as a safer, herbal-based adjunct in periodontal therapy, especially for patients seeking non-chemical or side-effect-free options for maintaining oral hygiene and preventing disease progression. How to cite this article: Gadekar AV, Dodamani AS, Vishwakarma PK, et al. Comparative Evaluation of Herbal Matcha Extract and 2% Chlorhexidine Gluconate on Periodontal Health Status: A Clinico-microbiological Study. J Contemp Dent Pract 2025;26(5):488-492.